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ISO 18363-2:2018

Animal and vegetable fats and oils -- Determination of fatty-acid-bound chloropropanediols (MCPDs) and glycidol by GC/MS -- Part 2: Method using slow alkaline transesterification and measurement for 2-MCPD, 3-MCPD and glycidol

SKU: iso_069575_166382 Published by ISO Publication Year 2018 1 Edition 21 pages

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This document specifies a procedure for the parallel determination of glycidol together with 2-MCPD and 3-MCPD present in bound or free form in oils and fats. The method is based on alkaline-catalysed ester cleavage, transformation of the released glycidol into monobromopropanediol (MBPD) and derivatisation of the derived free diols (MCPD and MBPD) with phenylboronic acid (PBA). Though free MCPD and glycidol are supposed to be present in fats and oils in low to negligible quantities only, in the event that free analytes are present, they would contribute proportionately to the results. The results always being the sum of the free and the bound form of a single analyte. This method is applicable to solid and liquid fats and oils. This document can also apply to animal fats and used frying oils and fats, but a validation study is undertaken before the analysis of these matrices. Milk and milk products (or fat coming from milk and milk products) are excluded from the scope of this document.

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GST REG No R119441681
QST REG No R1006017360

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ISO 18363-2:2018

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