Codes & Standards - Purchase
The method applies to organic compounds which, under the conditions of the test, are
- soluble in tap water at the test concentration and not expected to be transformed to insoluble metabolites if biodegradation, in addition to elimination, is determined;
- poorly water-soluble, but which are satisfactorily dispersible in water and allow detection with suitable analytical means (e.g. organic carbon measurements);
- non-volatile, or which have a negligible vapour pressure under the test conditions;
- not inhibitory to the test micro-organisms at the concentration chosen for the test. Compounds inhibitory at concentrations used in this test may be tested at concentrations less than their EC20 value, followed by higher practical concentrations after a period of acclimatization.
The method can also be used to measure the biodegradation and elimination of dissolved organic compounds in waste water (also called test compound in the method).